Day :
- Oral Sessions: Veterinary Medicine | Clinical Veterinary | Food Animal | Animal Welfare | Animal Models and Testing | Veterinary Care and Management
Chair
Ramesh C Gupta
Murray State University
USA
Co-Chair
Ahmed Mohammed Alluwaimi
King Faisal University
Saudi Arabia
Session Introduction
Hrefna Sigurjonsdottir
University of Iceland
Iceland
Title: Which variables affect most agonistic and affiliative behavior in pastured adult horses?
Time : 11:30-11:55
Biography:
Hrefna Sigurjonsdottir has completed her PhD from University of Liverpool, England. She has been affiliated as a University Teacher and Researcher at the University level in Iceland since 1982, doing research in the field of Animal Behavior and Pedagogy of Biology. For the last 10 years, she has studied horse behavior in pastures. She has published more than 20 papers in reputed journals and written educational material for schools in the field of biology. She has served on Editorial Board of the Icelandic Naturalist, been a Board Member of Landvernd, the Icelandic Environment Association for 10 years and recently she became a Member of the Board of the Animal Protection Association in Iceland.
Abstract:
Results from studies of social interactions (aggressive, submissive, allogrooming, play) in 17 groups of pastured Icelandic horses (329 in total) are used in multivariate analyses (PCA and GLM). The composition of the groups and conditions varied stability, group size, proportions of geldings/mares/sub-adults and presence of newborn foals, presence of stallions (6 groups) and more. Both individual and group characteristics determined the type of social interactions performed by each horse. Geldings were both more aggressive and submissive than either mares or stallions. Adult play was almost restricted to geldings. The higher the ratio of sub-adult to adults, the rates of aggression, play and allogrooming also gets higher. Group stability was highly influential on the frequency of the different social interactions. Both aggression and submission decreased with increasing stability, while allogrooming was more frequent in unstable groups and play in nearly stable groups. Such effect of stability was not observed in the breeding groups with a stallion. One major finding was that the frequency of social interactions, especially agonistic interactions, was much lower in the breeding groups than in other groups. These results are important in terms of understanding the determinism of social interactions. Their relevance for horse group management and horses’ welfare is also clear.
Liza Koster
University of Glasgow
UK
Title: Heart rate variability in dogs used in veterinary training facilities exposed to instrumental music
Time : 11:55-12:20
Biography:
Liza Sally Koster has completed her Bachelor’s degree, Master’s degree and Residency at the University of Pretoria, South Africa and obtained Specialist status in the European Board of Specialists in Internal Medicine. She has worked in referral and academic practice in Internal Medicine in 5 countries, including Senior Lecturer at the University of Sydney and Pretoria, Assistant Professor at Ross University School of Veterinary Medicine. She has recently commenced her appointment as Cardiorespiratory Clinician at the Small Animal Hospital, University of Glasgow. She has published more than 12 papers in reputed journals.
Abstract:
Heart rate variability (HRV) is the non-invasive method of measuring variation of R-R intervals of the ECG recording and is the final balance of the autonomic nervous system input on the sinoatrial node of the heart. HRV has been used to stratify risk of humans with cardiovascular disease including, coronary artery disease and elevated ST segment acute myocardial infarction. In dogs, HRV is reduced in those dogs with decompensated degenerative mitral valve disease. Music has benefited many captive animals in zoological institutions in terms of behavior and production animals in terms of productivity. Two studies have demonstrated that HRV has improved in shelter dogs in response to human interaction and classical music. There is a real concern regarding the welfare of dogs used by veterinary schools for teaching purposes. The aim of measuring HRV in dogs was to employ a non-invasive, portable method of measuring cardiovascular autonomic tone which could be viewed as a reflection of overall stress experienced by these dogs while they are being utilized in veterinary education. Sixteen kennel and student owned dogs were subjected to an observation cross-over study. Dogs were allocated to a Music or No Music category and after a 7-day washout period became their own controls. Time and frequency-domain variables were measured over a 80 minute period, unexpectedly mean heart rate variability (µRR) was lower in dogs that were exposed to auditory stimulation possibly due to novelty, while standard deviation 1 of Poincare plot (SD 1) was higher (p=0.94), standard deviation 2 of Poincare plot (SD2) was lower (p=0.4) and LF/HF was lower (p=0.03), all indicative of sympathetic withdrawal. The use of different genres and tempo of music needs to be explored further.
Bilal Cem Liman
University of Erciyes
Turkey
Title: Determination of acrylamide using immuno-enzymatic method in commercial dog and cat food
Time : 12:20-12:45
Biography:
Bilal Cem Liman has completed his undergraduate study in the Faculty of Veterinary Medicine of Ankara University in 1985 and joined PhD degree in the Department of Pharmacology-Toxicology, Faculty of Veterinary Medicine of Atatürk University in the same year and was assigned as a Research Fellow in 1986. He has completed his PhD degree in 1991. He was assigned as an Associate Professor in 1996 and as a full Professor in 2002. He has memberships in the following professional societies; Society for Veterinary Pharmacology-Toxicology (Turkey), Society for Toxicology (Turkey), EUROTOX and IUTOX. Within the framework of Socrates-Erasmus Academic Staff Mobility Program, he delivered undergraduate and postgraduate lectures in the Faculty of Veterinary Medicine of University of Udine in 2011. He has also served as Deputy Dean, Vice Dean and Head of the Department Pre-Clinical Science. He is currently working as a Faculty Member and Head of Department of the Pharmacology-Toxicology in University of Erciyes, Turkey.
Abstract:
Nowadays the pets which are grown in human auspices have no chance to choose their food and mistakes that people make about food of their cats and dogs will adversely affect the health of animals in a negative way. During the production of cat and dog food, high temperature applications at different stages are available. As s result of the starch containing structure of the cat and dog food, it is foreseen that acrylamides may be present in the food because of Maillard reaction. The aim of this study is to detect acrylamides in dry cat and dog food which are currently available in the market with a new method which is immunoenzymatic method. In this way potential health risks for cats and dogs will be detected, for the first time using this method acrylamides levels will be detected and applicability of this method is going to be tested. For this purpose, from a variety of firms in the market 42 cats and dogs food were randomly collected and passed through several stages for testing. Residual acrylamide in food was tested using immunoenzymatic method and as a result analyzed dry cat and dog food samples, 33% (14 samples) presence of acrylamide have been found, in these samples average acrylamide density is 87 ppb. According to the findings the highest acrylamide density is 155 ppb and the lowest is 45 ppb, in 67% of total samples acrylamide was not detected. The values set out in findings are way below from European Commission (EC), the European Food Safety Authority (EFSA) assessment of human food indicator value. Consequently, immunoenzymatic method can be used as a standard technique on cat and dog food but it must be supported by a wider range of research. Again it can be advised that while preparing food for pets without the chance of food selection formulation changes, process parameter changes and specially extrusion and other heat related stages can be done more carefully to limit Maillard reaction which causes acrylamide in food.
Vishnu Sunil Jaikumar
Rajiv Gandhi Centre for Biotechnology
India
Title: Efficacy of bovine collagen 10% alginate dressing on cutaneous wound healing in dogs
Time : 12:45-13:10
Biography:
Vishnu Sunil Jaikumar had completed his Masters in Veterinary Surgery and Radiology from College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Kerala, India. He is currently working as Research Associate in Rajiv Gandhi Centre for Biotechnology, Kerala, India. Formerly, he worked as Veterinary Surgeon in an institution named Animal Birth Control for Dogs (ABCD) KOCHI run by Kochi Municipal Corporation, the first government run facility solely for the purpose of control of stray dog population in Kerala, India. He is an ardent animal lover and is planning to pursue his PhD abroad.
Abstract:
The study was conducted in twelve dogs with clinical signs of full thickness non healing cutaneous wounds brought to the University Veterinary Hospitals of Mannuthy and Kokkalai, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, India to evaluate and compare the efficacy of bovine-collagen and bovine collagen - 10% alginate sheets in canine skin wound healing. The dogs were randomly divided into two groups viz., a pre-sterilized bovine collagen - 10% alginate sheet (Group-I) and a pre-sterilized bovine collagen sheet (Group-II), on wound surface after thorough surgical debridement under general anesthesia. Re-application of the dressings was carried out at 48 hours intervals for three times followed by weekly bandaging. Wound attributes were studied on the day of presentation and 7th, 14th and 28th days post-application. Skin punch biopsy taken from representative samples on day 28 were subjected to Gomori’s one step trichrome staining to study the extent of collagen deposition and orientation of fibres, fibroblast proliferation and regeneration. The study revealed no significant difference in the efficacy between bovine collagen - 10% alginate and bovine collagen sheets in full thickness cutaneous wound healing in dogs. Both the collagen sheets were well tolerated by the patients with no adverse reactions and healing could be observed within 28 days with near to normal histomorphology. However, addition of 10 percent alginate in bovine collagen dressings could achieve absorption of exudates significantly early, higher rates in reduction of wound surface area, wound contraction and epithelialization.
Hatice Ozlem Nisbet
Ondokuz Mayis University
Turkey
Title: The influence of 900 to 1800 Mhz electromagnetic field on testicular function and structure of growing rats
Time : 14:45-15:10
Biography:
Hatice Ozlem Nisbet has received her DVM from Uludag University, Faculty of Veterinary Medicine in 1990 and her PhD from Uludag University, Faculty of Veterinary Medicine, Department of Surgery in 1997. She is a Researcher and Lecturer in Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Surgery, Samsun, Turkey. She has authored/co-authored over 35 articles in scientific journals.
Abstract:
The purpose of this study was to evaluate the possible effects of whole body electromagnetic field (EMF) exposure on reproduction in growing male rats. Male albino Wistar rats (2 days old) were exposed to EMF 1800 and 900 MHz for 2 hours continuously per day for 90 days. Sham control was kept under similar conditions except that the field was not applied for the same period. After blood samples were collected, the animals were sacrificed 24 hours after the last exposure and the tissues of interest were harvested. The mean plasma total testosterone showed similarity among the two study groups and was significantly higher than the sham control rats. The percentage of epididymal sperm motility was significantly higher in the 1800 MHz group (p<0.05). The morphologically normal spermatozoa rates were higher and the tail abnormality and total percentage abnormalities were lower in the 900 MHz group (p<0.05). Histopathologic parameters in the 1800 MHz group were significantly higher (p<0.05). In conclusion, the present study indicated that exposure to electromagnetic wave caused an increase in testosterone level, epididymal sperm motility (forward) and normal sperm morphology of rats. As consequences, 1800 and 900 MHz EMF could be considered to be a cause of precocious puberty in growing rats.
Sila Daniel Damwesh
Nakam Memorial School
Nigeria
Title: Biotechnology for animal (Poultry) production in a changing world: A must for developing nations
Time : 15:10-15:35
Biography:
Sila Daniel Damwesh has completed his Master’s degree in Animal Production and Range Management with distinction in Research in 2012 from Modibbo Adama University of Technology, Nigeria. In his first degree he was awarded Chief (Mrs) Dorcas Alo’s Prize for the Best Final Year Project in Animal Science. He has been an Instructor in Biology at Nakam Memorial School, Nigeria. He has published 4 papers in reputed journals and he is a Member of Animal Science Association of Nigeria (ASAN) and was a Supervisor of International Young Inventors Competitions (IEYI, IIDC) in Indonesia, Thailand and Taiwan.
Abstract:
Biotechnology is widely used in animal production with numerous other potential applications. It can be applied for improving animal performance through better nutrition, enhanced production potential or improved health status. Plant biotechnology can produce crops which are embedded with improved nutritional value or incorporate vaccines or antibodies into feeds that will cheaply and effectively protect the animals against diseases. Transgenic manipulation of commensal gut or rumen microorganisms has considerable potential for improving nutrition, gut development and health in animals. Accelerated growth and leaner carcasses in meat animals and increased milk production in dam cows is achieved by the administration of recombinant somatotropin (SI) and by controlling the expression of the gene with ad hoc promoters thereby preventing severe impairment of the health status of transgenic animals. The paper also unfolds some biotechnologically generated possibilities through the production of genetically modified (GO) crops, value-added traits, added-value feed stuffs (quality traits) as well as the production of dietary enzymes and production of transgenic animals for use in animal production with particular bias on poultry. Animal breeding is another application. While developed nations are leading the way, developing countries are lagging behind due to a number of factors such as lack of man power, poor infrastructure /laboratories, misplaced priorities and lack of self will by both the Government and Management of the various institutions in developing nations. A drastic adjustment and redemptive action by developing and developed nations respectively is suggested as a way forward.
Palanisamy Sankar
Tamil Nadu Veterinary and Animal Sciences University
India
Title: Effect of nanocurcumin for management of subclinical mastitis
Time : 15:35-16:00
Biography:
Palanisamy Sankar, budding Pharmacologist, works as Assistant Professor in the Department of Veterinary Pharmacology and Toxicology, Veterinary College and Research Institute, under Tamilnadu Veterinary and Animal Sciences University (TANUVAS), Chennai. He got his BVSc in Veterinary Science at TANUVAS in the year 2005, MVSc and Doctoral degree in Veterinary Pharmacology and Toxicology in the year 2008 and 2012 respectively at Indian Veterinary Research Institute, Deemed University, Izatnagar, Bareilly, India, where he worked as a Scientist for four years in the area of Toxicology and Nanoparticle drug delivery. Dr. P. Sankar was awarded with ICAR Junior Research Fellowship for pursing his Masters Degree and Senior Research Fellowship for pursing his Doctoral Programme. Currently his research focuses on the nanoparticle drug delivery system to improve the drug efficiency of existing drugs in markets. In addition, He had completed three research projects in the area of nanoparticle drug delivery and Toxicology and has published research papers in the peer reviewed international journals with good impact factor.
Abstract:
The therapeutic potential of polymeric nanoparticle-encapsulated curcumin was evaluated in mouse model of mastitis. Mastitis caused by Staphylococcus aureus is usually subclinical and chronic in nature. Poly-(D, L-lactide)-co-glycolide (PLGA)-encapsulated curcumin nanoparticles (CUR-NP) prepared through solid-in-oil-in-water emulsion technique were administered by oral gavage as pre-treatment from day 2 to day 7 of parturition. Both curcumin and CUR-NP were administered at 100 mg/kg bw. Mastitis was induced by infecting the mice with Staphylococcus aureus through intramammary inoculation on the 9th day of parturition. Accordingly, the curcumin or CUR-NP-pretreated mice were given intramammary inoculation. Body temperature was recorded at different time intervals after inoculation. Mammary tissues from animals were collected at 24, 48 and 72 hours post-infection. There was swelling in the mammary gland of the mastitis control mice. In these animals, there were significant rise in body temperature and increase in neutrophil and decrease in lymphocyte counts. The swelling subsided in both the curcumin- or CUR-NP-treated mice after 12 to 24 hours, while body temperature and the leukocyte counts were restored after 48-72 hours in these animals. The number of colony forming unit (CFU) counted in the L4 abdominal mammary gland homogenate of the mastitis control group was significantly reduced with both curcumin and CUR-NP. Differential bacterial count was done in the same homogenate. Curcumin significantly decreased the total and extracellular counts, whereas CUR-NP also decreased intracellular count. Comparison of the effects showed that CUR-NP was significantly more effective in reducing the body temperature, CFU and intracellular bacterial count than curcumin. These results suggest that CUR-NP may possess better potential in alleviating murine mastitis than curcumin.
Mohammed Baba Ardo
Modibbo Adama University of Technology
Nigeria
Title: A six months survey of gross pathological conditions of slaughtered cattle at Yola Abattoir Adamawa State, Nigeria
Time : 16:15-16:40
Biography:
Mohammed Baba Ardo has completed his DVM degree from University of Maiduguri, Nigeria and completed his Masters in Preventive Veterinary Medicine at the University of Ibadan, Nigeria. He has obtained his PhD in Veterinary Public Health (2009) from the University of Nigeria. He is the Head of Department, Animal and Range Management, Modibbo Adama University of Technology, Yola, Nigeria. He has published over 30 papers in reputed journal.
Abstract:
A cross sectional study was conducted from November, 2014 to April, 2015 on 14,508 slaughtered cattle at Yola Abattoir Adamawa State on Postmortem examination. The objectives of the study were to determine the prevalence of Contagious Bovine Pleuro Pneumonia (CBPP), Bovine Fasciolosis and Bovine Tuberculosis (BTB) in slaughtered cattle according to sex and breeds. A regular visit was made to the Yola Abattoir at 6:00 AM seven times a week and this was done for six months. A total of 14,508 slaughtered cattle were examined at postmortem and 3,418 (23.56%) cases were recorded; CBPP 898 (6.19%), Fasciolosis 1,100 (7.58%) and TB 1,420 (9.79%). There was statistical significance (P<0.05) in the distribution of the diseases among the slaughtered cattle. However, according to sex out of the 3,418 cases, 908 (6.26%) were male while 2,510 (17.30%) were female. There was high significance difference in sex distribution of the diseases and in terms of breeds, Adamawa Gudali 1,006 (6.93%), Sokoto Gudali 900 (5.51%), Red Bororo 800 (6.20%) and White Fulani 712 (4.91%). There was statistical variation (P<0.05) among the breeds. This study would serve as a valuable tool in monitoring pathological condition in the study area.
M Younus
University of Veterinary & Animal Sciences
Pakistan
Title: Hematological changes in the hydatidosed male sheep after experimental inoculation of Echinococcus granulosus eggs
Biography:
M Younus is currently working at Directorate of Advance Studies and Research, University of Veterinary and Animal Sciences, Pakistan. His area of expertise includes livestock & dairy farm management.
Abstract:
A total of 48 apparently healthy weaning sheep lambs (Ovis aries) of 8-10 weeks old weighing within the range of 7-10 Kg were purchased from the contractors maintained in the experimental station of University of the Punjab, Quaid-e-Azam Campus (New Campus) at Lahore, Pakistan. They were deformed against nematodes (Round worms) with levamisol (ICI) at the dose rate of as per the direction of the manufacturers. The fecal examination was tested against the parasitic eggs no helminthes ova were seen. All the 48 sheep lambs (kids) were divided into two groups i.e., group A & group B. Group “A” was comprising of 40 sheep and kept as infected groups whereas group “B” was comprising of eight (8) sheeps & kept as new infected control group. Each sheep kid of infected group A was given 3-4 fresh gravid segments contains 2-3 thousands eggs of Echinococcus granulosus which were collected from experimentally infected dogs by feeding fresh hydrated cysts collected from liver & lungs of sheep after slaughtering process. Each sheep kid of infected dog was fed fresh gravid segments for a total period of 5 days or each alternate day. Coagulated blood was collected before the start of oral administration of infection and after every month by jugular phlebotomy of each sheep kid before the infected & new infected control group. One sheep lambs each from rejected & now infected group was slaughtered at the end of each month for the presence of macroscopic hydrated cyst in the visceral and abdominal cavity. After 180 days of the experiment the hydrated cysts were confirmed in the abdominal cavity. Hematological parameters or zero day i.e., just before the administration of the gravid segments to the lambs and then at the end of every month revealed that there was a gradual increase (PL 0.05) in the White Blood Cell (WBC), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin Concentration (MCHC) and Erythrocyte Sedimentation Rate (ESR). The increasing trend was probably due to inflammatory response and lytic effect of the newly developing E. granulosus hydrated cysts. The Red Blood Cell (RBC), Hemoglobin (HB), Packed Cell Volume (PCV) and Mean Corpuscular Hemoglobin (MCH) infected groups were decreased significantly as compared to the control group (PL 0.05). The experiment was terminated at the end of the 7th month.
Christie Peacock
Sidai Africa Ltd
Kenya
Title: Sidai Africa Ltd: An innovative approach to the delivery of quality livestock services in Africa
Biography:
Christie Peacock is the Founder and Executive Chairman of Sidai Africa Ltd., a social enterprise in Kenya offering quality livestock services through a network of branded franchises. Sidai Africa Ltd. is a Kenyan company with start-up financing from the Bill & Melinda Gates Foundation and is owned by the international NGO FARM-Africa. Christie is an animal scientist and former Chief Executive of FARM-Africa. She has worked in agricultural development in Africa and South East Asia for over 30 years. She is a founding member of the All Party Parliamentary Group for Agriculture and Food for Development and is a member of the Guardian newspaper’s Global Development website Advisory Panel. Dr. Peacock was awarded an Honorary Doctorate from the University of Reading and selected as an Ashoka Fellow in 2011.
Abstract:
Livestock farmers in Africa struggle to access good quality inputs, effective knowledge and fair markets. In most countries the government veterinary service is under-resourced and unable to provide a full range of services to livestock keepers. Regulation of the emerging private sector is very weak allowing unqualified people to sell livestock products, including Prescription Only Medicines (POMs) directly to farmers. Many of these products are of sub-manufacturing standard, or are counterfeits. This leads to extensive misuse of drugs through poor diagnosis, incorrect product use and under-dosing. Drug resistance is rising in anthelmintics, antibiotics, acaricides and trypanocides. Sidai Africa Ltd is a pioneering company that aims to open access to good quality livestock services to farmers and pastoralists in Kenya. It is setting up a national network of professionally-managed branded Livestock Service Centres across Kenya which only stock quality products and offer a full range of livestock services. Sidai places emphasis on herd health, preventing disease and improving management wherever possible. Sidai’s buying power is already improving the profitability of Sidai centres enabling them to offer professional services including clinical services, AI, vaccination, simple diagnostics and farmer training. In 3 years Sidai has opened 120 centres serving 100,000 farmers in Kenya. Sidai centres are run by technically-qualified professionals who may be Sidai staff or business partners operating as franchisees using the Sidai brand.
Aavash Koirala
Institute of Agriculture and Animal Sciences
Nepal
Title: Spaying and neutering of dogs in control of rabies
Biography:
Aavash Koirala is currently a student of IAAS Rampur, Nepal. He has worked as a Communication Officer for One Health Nepal and conducted FMD vaccination program and rabies mass vaccination and awareness campaign. He has also worked as a Program Coordinator for “Basic Small Animal Soft Tissue Surgery and Anesthesia Course†held on 4th-19th April 2016 organized by WVS ITC Thailand, India and Hart with collaboration of AFU.
Abstract:
Spaying and neutering are term used to describe surgical removal of reproductive organs of female and male animals respectively. Over many years ago, the procedure would be considered cruel by today’s standards. Technique to sterilize livestock had already been established but spaying and neutering was not accessible for dogs till 1930. In 1964, first low cost spay neuter clinic was opened in Los Angeles. Now every year on the last Tuesday of February, world spay day is celebrated. Recently new project “manumitra†has been launched by Kathmandu Metropolitan City in association with Human Society International and Jane Goodall Instiute, Nepal for streets dog management program. This surgical procedure is divided into 3 division; pre-operative, operative and post-operative. Common uses drugs in spaying and neutering are Xylazine, Anesthesia (solution of Ketamine or Diazepam), Meloxicam, Megapen, Ivermectin, Lignocaine for male only and suture material used are chromic catgut and PGA. Rabies is one of the fatal viral diseases caused by the bite of infected dogs. Especially the street dogs are highly susceptible to infection by rabies. As in six years, one female dog and her offspring can theoretically produce 67,000 dogs. Hence to minimize the chance of rabies infection to the human, spaying and neutering is the best long term strategy. Carrying this very concept many organization has started spaying and neutering practices. Recent advancement shows that there is new way of sterilization by using injectable chemical. Different project has been launched in Nepal for controlling rabies in associated with the slogan, “no babies no rabiesâ€.
- Symposium
Session Introduction
Ramesh C Gupta
Murray State University
USA
Title: Recent developments in toxicity and treatment of organophosphates and carbamates
Time : 10:35-11:15
Biography:
Ramesh C Gupta has obtained his DVM, MVSc and PhD from India and currently serves as Professor and Head of Toxicology Department. For decades, he has conducted experimental brain research in relation to pesticide toxicity. He has made presentations in Australia, Italy, Japan, Germany, France, Switzerland, Spain, Czech Republic, Sweden, South Korea and China. He has served the panels of NIH, CDC, NIOSH and NAS and has published >350 publications, including 7 books: Toxicology of Organophosphate & Carbamate Compounds, Veterinary Toxicology, Handbook of Toxicology of Chemical Warfare Agents, Anticholinesterase Pesticides, Reproductive & Developmental Toxicology, Biomarkers in Toxicology and Nutraceuticals. In 2006, he has received the Murray State University's Distinguished Researcher Award. He is a Diplomate of American Board of Toxicology and Fellow of American College of Toxicology, American College of Nutrition and Academy of Toxicological Sciences.
Abstract:
Currently, there are more than 250 synthetic organophosphate (OP) and two dozen carbamate (CM) compounds that are commonly used in agriculture and human and animal health protection; in addition, those compounds are also involved in malicious and suicidal intents. As a result of their extreme toxicity, lack of species selectivity and worldwide use, OPs and CMs continue to pose serious threats to animal and human health. In the current world situation, the biggest threat is from the use of OP nerve agents. Depending upon the dose and duration of exposure, OPs can cause acute or chronic toxicity. More than a dozen OP pesticides also produce an intermediate syndrome, which is a separate clinical entity from acute and chronic toxicity. OPs primarily affect the nervous system and skeletal muscles and cause hypercholinergic preponderance due to acetylcholinesterase (AChE) inactivation at the synapses and neuromuscular junction. Some OPs and CMs directly interact with cholinergic receptors. In addition to cholinergic elements, they interact with many other critical molecules, including receptors (NMDA, cannabinoids and others) and enzymatic and non-enzymatic proteins. OPs have complex chemical structures (>13 types) and with varying affinities, they interact with the above molecules and each OP produces a different toxicological profile. OPs and CMs exert oxidative/nitrosative stress, which is involved in neurotoxicity, neuroinflammation, dendritic damage and other toxic effects. Biomarkers of exposure (OPs, CMs and/or their metabolites), effects (at molecular, cellular, morphological, inflammation and behavioral levels), and susceptibility (cytochrome P-450, PON-1) to OPs have been proposed but many of them have yet to be validated. Because of the complex mechanisms involved in the toxicity of OPs, treatment with atropine sulfate and 2-PAM is not always successful. Therefore, in recent years, novel oximes have been developed that seem to be quite effective against certain types of OPs, such as nerve agents. To date, treatment of CM poisoning rests with atropine sulfate. Current efforts in the area of OP/CM research are to explore the novel toxicological mechanisms, identify the most sensitive and quantifiable biomarkers and develop a universal antidote.
- Special Session
Session Introduction
Olivier Sparagano
Coventry University
UK
Title: Poultry parasites: Emerging issues
Time : 12:15-13:00
Biography:
Olivier Sparagano has completed his PhD in 1994 on Primary Amebic Meningo-encephalitis at the Institut Pasteur in Lyon, France. He has worked on Animal Health related issues for the last 25 years. He is currently the President of the Society for Tropical Veterinary Medicine and the Chair of a European Network (COREMI) linking 28 countries to control the poultry red mite, Dermanyssus gallinae. He has published more than 140 scientific papers in peer-reviewed journals and is also a Fellow of several organizations.
Abstract:
The poultry red mite (PRM) Dermanyssus gallinae is a blood-feeding ectoparasite, well known as a threat to the poultry industry; adversely affecting production and hen health and welfare throughout the globe (with an average prevalence around 80-90% in many European countries and worldwide), both directly and through its role as a disease vector. The poultry red mite can feed and reproduce very quickly, difficult to be reached when control methods are used on farms or even in human dwellings. Veterinarians and medical practitioners are struggling to find appropriate prevention and control methods to reduce its impact. PRM can lower egg production, egg quality, animal welfare, profit margins and increase risks of disease transmission such as Salmonellosis. This paper will present the different biological, chemical and physical methods used to control such pest. However, acaricide resistance, new EU regulations (enriched cages and beak trimming for instance) and chemical withdrawal from commercial companies are making its proliferation much easier. New approaches based on vaccine production and/or genomic analyses have created opportunities to address new ways to attack the survival and development of such parasitic arthropod. However its rapid and prolific life cycle, resilience to environmental condition changes and genetic diversity makes early interventions a key aspect in its control. This paper will also explain the development of a new European initiative based on a Cost Action network (FA1404) being currently running across 28 countries.
- Oral Sessions: Veterinary | Veterinary Research | Animal Reproduction and Genetics | Avian and Exotics
Chair
Olivier A E Sparagano
Coventry University
UK
Co-Chair
Jonathan L Heeney
University of Cambridge
UK
Session Introduction
Ramesh Gupta
Murray State University
USA
Title: Safety evaluation of ectoparasiticides in dogs using GC/MS
Time : 14:00-14:25
Biography:
Ramesh Gupta has earned his DVM, MVSc and PhD from India and currently serves as Professor and Head of Toxicology Department. For decades, he has conducted experimental brain research in relation to pesticide toxicity. He has served the panels of NIH, CDC, NIOSH, and NAS and has published more than 350 publications, including 7 books: Toxicology of Organophosphate & Carbamate Compounds, Veterinary Toxicology, Handbook of Toxicology of Chemical Warfare Agents, Anticholinesterase Pesticides, Reproductive & Developmental Toxicology, Biomarkers in Toxicology and Nutraceuticals. In 2006, he has received the Murray State University's Distinguished Researcher Award. He is a Diplomate of American Board of Toxicology and Fellow of American College of Toxicology, American College of Nutrition and Academy of Toxicological Sciences.
Abstract:
Currently, the global dog population is estimated at 900 million (>80 million in the US, 15.9 million in Russia, 8.5 million in the UK, 7.3 million in France, 7 million in Italy and 6.9 million in Germany). Dogs are commonly infested with blood sucking ticks, fleas, mosquitoes and other ectoparasites which pose a serious global health concern as they transmit infectious diseases between humans and animals. Therefore, the use of ectoparasiticides on dogs is crucial and inevitable. Presently, a large number of ectoparasiticides (having a combination of active ingredients) are used to provide broad spectrum effects against ectoparasites as larvicides and adulticides. Due to lack of safety data, concerns have been raised for their safe use in dogs and the risks posed to those that handle the dogs on a daily basis. Using GC/MS, residues of fipronil, s-methoprene, selamectin, etofenprox, amitraz, permethrin, cyphenothrin, pyriproxyfen, indoxacarb and piperonylbutoxide were determined in cotton glove extracts in variable concentrations after topical application of various ectoparasiticides (Frontline, Advantage, Revolution, Bio Spot Defense, Certifect, Activyl, Parastar Plus and Vet Guard Plus). GC/MS analysis offered identification, confirmation based on specific ions and quantitation of each pesticide in glove extract to assess the level of residue transfer and risk to humans. Dog blood analysis revealed no residue of any pesticides, suggesting poor dermal absorption. None of the products caused any adverse effects to the dogs. Owners and veterinary personnel can be exposed to significant levels of pesticides following daily exposure if proper precautions are not taken.
Shengqing Yu
Shanghai Veterinary Research Institute-Chinese Academy of Agricultural Sciences
China
Title: Riemerella anatipestifer AS87_01735 gene encodes nicotinamidase (PncA), an important virulence factor
Time : 14:25-14:50
Biography:
Shengqing Yu has completed her PhD in 2002 from Tottori University, Japan and Postdoctoral studies from National Institutes of Health, USA. She is the Head of Veterinary Public Health Department, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. She has published more than 60 papers in reputed journals.
Abstract:
<i>Riemerella anatipestifer</i> is a major bacterial pathogen that causes septicemic and exudative diseases in domestic ducks. In our previous study, we found that deletion of AS87_01735 gene significantly decreased the bacterial virulence of <i>R. anatipestifer</i> strain Yb2 (mutant RA625). AS87_01735 gene was predicted to encode nicotinamidase (PncA), a key enzyme that catalysis conversion of nicotinamide to nicotinic acid, an important reaction in the NAD+ salvage pathway. In this study, the AS87_01735 gene was expressed and identified as a PncA coding gene using enzymatic assay. Western blot analysis demonstrated that <i>R. anatipestifer</i> PncA was located in the cytoplasm. The mutant strain RA 625 (named as Yb2ΔpncA in this study thereafter) showed similar growth rate, but decreased NAD+ quantities in both exponential and stationary phases in TSB culture when compared with wild-type strain Yb2. In addition, Yb2ΔpncA infected ducks showed much less bacterial loads in the blood, and no visible histological changes in heart, liver and spleen. Furthermore, Yb2ΔpncA immunization conferred effective protection in ducks against the virulent wild-type strain Yb2 challenge. Our results suggest that <i>R. anatipestifer</i> AS87_01735 gene encodes PncA, which is an important virulence factor and the mutant Yb2ΔpncA could be used as a novel live vaccine candidate.
Srebrenka Nejedli
University of Zagreb
Croatia
Title: Organogenesis of larvae trout (Salmo thymusobtrusirostris, Heckel, 1851) from Vrljikariver, Croatia
Time : 14:50-15:15
Biography:
Srebrenka Nejedli is currently a Professor in Department of Anatomy, Histology and Embryology, Veterinary Faculty, University of Zagreb, Croatia. She is a Member of EAVA and European Aquaculture Society.
Abstract:
Investigations were carried out on trout larvae (Salmo thymusobtrusirostris, Heckel, 1851) age of 10-56 days caught from Vrljika river, Croatia. Larvae were taken every two days from 10 to 50 days and 56 days and were stained with Hematoxylin and Eosin (HE), Periodic Acid-Schiff reaction, Toluidin Blue (TB), Alcian Blue-Specific (pH=2.5) (AB), Alcian Blue and Alizarin Red. Organogenesis of the larvae were analyzed, primarily the development and morphology of muscle tissue, osteological development and development of digestive tract. The number of myomeres in dorsal epaxial musculature on the larvae was measured and it was on 10-18 days 42.17, 20-28 days 50.40, 30-38 days 55.65, 40-48 days 56.92 and 50-56 days 58.90 myomeres. Total length (TL) increased from minimal length of 19.57 mm on 10-28 days to maximal TL 25.80 mm on 50-56 days. Number of vertebrates in all investigated groups was 59. There was no sign of ossification in the vertebral column or in the fins and in the head bones. Growth of larval to complete yolk sac absorption takes in the age about 24-26 days when the animals pass through the opening of the mouth. In all investigation, larvae were not skeletal malformations.
Ahmed Alluwaimi
King Faisal University
Saudi Arabia
Title: The CD markers of the camel (Camelus dromedarius) mammary glands in health and disease
Time : 15:15-15:40
Biography:
Ahmed Mohammed Alluwaimi has completed his PhD from the University of Birmingham, UK in 1994 and his MSc from Cornell University, Ithaca, Newyork in 1986. He has done his Postdoctoral studies at University of California, Davis, USA and Monoclonal Antibody Center and Flowcytometry Facility, Washington State University, Pullman, USA. He is the Director of Saudi Veterinary Medical Society and the General Secretory of Almari Award for Veterinarian. He has published more than 25 papers in reputed journals. His major research interest is to study the nature of the immune responsese in camel infected with Mycobacterium avium subspecies paratuberculosis (MAP) and the immune system of the camel mammary glands.
Abstract:
Dromedary camel (Camelus dromedarius) is one of the highly valuable domestic animals in Saudi Arabia. Camel is multipurpose animal that can be used for meat, milk and wool production. Lymphocyte trafficking to the mammary glands is under the influence of the adhesion molecules (AM). Expression of the AM such as selectins, for e.g., CD62L and integrins, like mucosal address in cell-adhesion molecule-1 (MAdCAM-1) dictate the type of the recruited cells and their homing destination. Therefore, AM expression is vital in determining the mammary glands cellular populations in health and disease. The nature of the immune responses in the camel mammary glands is not understood completely. There are very few studies on the cellular populations of the camel mammary glands and the lymphocytes trafficking in health and disease. Our studies on the expression and distribution of CD markers and the adhesion molecules, in the alveolar tissues, supra-mammary lymph nodes, Peyer's patches and mesenteric lymph node of the healthy camels were explored at lactating and lactating period indicated that CD8+ T-cells were detected in both mammary alveolar tissues and the supra-mammary lymph nodes with the highest expression observed in the lactating period. WC+1+ expression on ï§ï¤ cells were evident in mammary tissues and supra-mammary lymph nodes at both stages. MAdCAM-1 was detected in almost all the tissues at the two physiological stages in which high expression was evident in the non-lactating period. Flow cytometry study of the camel milk cellular population at mid lactation has detected the high expression of CD3+, CD8+, WC+1+ and LPAM-1. The expression of the LPAM-1 on the lymphocytes and the intensive expression of the MAdCAM-1 in the camel mammary glands tissues have provided further support to the notion that the lymphocytes trafficking to the camel mammary glands could be of mucosal nature. Camel milk cells from Gram-positive and Gram-negative infected camels were examined with flow cytometry using cross-reacting antibodies like, anti-CD4+, CD8+, WC+1+ï§ï¤, CD62L, CD11a+/CD18, LPAM-1 and CXCR2. The mean percentage of the expressed CD markers has shown that CD62L, CXCR-2, LPAM-1, CD11a/CD18, CD8+, IL-6R and CD20+ were expressed in significant differences in either type of the infection. The LPAM-1 expression has provided further support to the notion that the lymphocyte trafficking is of the mucosal nature. The mucosal origin of cellular trafficking has important implications on the vaccine design and therapeutical approaches to mastitis.
P Sankar
Tamil Nadu Veterinary and Animal Sciences University
India
Title: Effects of novel water soluble nanocurcumin on arsenic-induced genotoxicity in rats
Time : 15:40-16:05
Biography:
P Sankar is currently working as an Assistant Professor in the Department of Veterinary Pharmacology and Toxicology, Veterinary College and Research Institute, under Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai. He has obtained his BVSc in Veterinary Science at TANUVAS in the year 2005, MVSc and Doctoral degree in Veterinary Pharmacology and Toxicology in the year 2008 and 2012 respectively at Indian Veterinary Research Institute, Deemed University, India, where he worked as a Scientist for four years in the area of Toxicology and Nanoparticle Drug Delivery. He was awarded with ICAR Junior Research Fellowship for pursing his Master’s degree and Senior Research Fellowship for Doctoral Program. Currently his research focuses on the nanoparticle drug delivery system to improve the drug efficiency of existing drugs in markets. In addition, he has completed three research projects in the area of nanoparticle drug delivery and toxicology and has published research papers in the peer reviewed international journals with good impact factor.
Abstract:
Curcumin loaded PLGA nanoparticles (CUR-NP) were synthesized to explore whether nanoformulation of curcumin can cause better protective effect than free curcumin against arsenic-induced genotoxicity in rat model. The CUR-NP prepared by emulsion technique was spherical in shape with particle size of 130.8 nm and 86.5% entrapment efficiency. The CUR-NP showed biphasic release pattern and it was dissolved in water easily. Rats were randomly divided into five groups of six each. Group I was kept as the control. In Group II, rats were exposed to sodium arsenite (25 ppm) daily through drinking water for 42 days. Groups III, IV and V were treated with arsenic as in Group II, however, they were administered empty nanoparticle, curcumin (100 mg/kg bw) and CUR-NP (100 mg/kg bw), respectively, by oral gavage during the last 14 days of arsenic exposure. At term, bone marrow cells were collected. The result indicated that arsenic significantly increased the formation of chromosomal aberrations, micronuclei and DNA damage in rat bone marrow cells. Free curcumin and CUR-NP treatment significantly attenuated these arsenic-mediated genotoxicity. However, the magnitude of the effects indicates that CUR-NP has better protective effect than free curcumin at the equivalent dose level for preventing arsenic-induced toxicity.
De Bastiani Grasiela
Universidade Federal de Santa Maria
Brazil
Title: Histochemistry of equine damaged tendons and ligaments
Biography:
De Bastiani Grasiela has completed her MSc from Universidade Federal de Santa Maria and Postdoctoral studies from Universidade Federal de Santa Maria, Brazil. She is specialized in diagnostic imaging of the horses and Professor of Imaging Diagnostic.
Abstract:
The repair process of tendons and ligaments includes different phases such as inflammation, neovascularization, proliferation of granulation tissue and fibrosis tissue. Collagen type and tissue characteristics of tendon and ligament repair are described. Samples of equine tendons and ligaments specimens of the metacarpophalangeal joint region were evaluated by ultrasonography and macroscopicaly and prepared for routine histopathology. The Masson’s trichromic and pricosirius staining techniques were also performed. Ligaments and tendons pathologic findings included fibroplasia, collagenolysis, cartilaginous metaplasia and lymphohistioplasmacytic inflammation. Tendons and ligaments scars were composed predominantly of type III collagen but there also was some type I collagen. Fiber alignment of tendons and ligaments in the reorganization tissue was not perfect and the appearance was characterized by a lack of the fiber crimp and parallelism. The microscopic and histochemistry staining techniques allowed the characterization of fiber alignment, collagen type, inflammatory cell infiltration and neovascularization that happens during the repair process of tendons and ligaments. These findings at different moments of the repair process were associated with ultrasonographic and anatomopathological findings.
Sulaiman Depamede
University of Mataram
Indonesia
Title: Pancreatic adenocarcinoma up-regulated factor (PAUF) in the saliva of Bali cattle (Bos javanicus): A biomarker candidate for infections
Biography:
Sulaiman Depamede has completed his PhD from The University of Adelaide, South Australia. Besides being a Lecturer at the Faculty of Animal Science of Mataram University, he is also a Chairman of Immunobiology Laboratory, Mataram University (2005-2015). He is currently serving as a Secretary of the Consortium for Research Large ruminants. His research in the last five years focused on the proteomics of Bali cattle (Bos javanicus) saliva. In 2013, he was a Visiting Scientist at the Ruakura Research Centre, Hamilton, New Zealand. He has published his research papers in reputed journals.
Abstract:
The aim of this study was to explore the potential of a peptide, pancreatic adenocarcinoma up-regulated factor (PAUF) expressed in the saliva of Bali cattle (Bos javanicus), which was first reported by Depamede (2013), as a biomarker for infections. For this purpose, a polyclonal antibody against PAUF peptide was produced using a synthetic PAUF peptide. This was synthesized from the PAUF amino acid sequence as the results of MALDI-TOF/TOF.MS and bioinformatics analysis of the Bali cattle saliva. Antibodies obtained were used to analyze for the presence of PAUF in Bali cattle (Bos javanicus) saliva as well as in the saliva of the New Zealand dairy cows (Bos taurus). The results showed that PAUF is present at greater abundance in Bali cattle saliva compared to that of Bos taurus. Interestingly, PAUF was found in the milk of infected New Zealand cows (Bos taurus), suggesting that it could also be a marker for mastitis. These results provide a novel potential breed and disease biomarker that could help in better management of Indonesian cattle. These findings still need to be further investigated for their utility as biomarkers as well as the role they play in physiology of the immune response in Bali and New Zealand cattle.
- Young Researchers Forum
Chair
Jonathan L Heeney
University of Cambridge
UK
Session Introduction
Jan Selfridge
McTimoney College of Chiropractic
UK
Title: When it comes to back care, size really doesn’t matter, In African safari elephants, does carrying a rider or multiple riders’ impact on musculoskeletal integrity?
Time : 16:20-16:40
Biography:
Selfridge Jan has completed her Postgraduate training at the McTimoney College of Chiropractic to earn a Masters’ degree in Animal Manipulation. Her practice covers South Oxfordshire, South Buckinghamshire and occasionally South Africa. Her lifelong passion for the protection and greater understanding of elephants led her to apply knowledge gained through her first degree in Animal Behavior & Welfare and her MSc studies to explore the impact of riders on elephants as their survival becomes increasingly dependent in the tourism industry. Her smaller equine, canine and feline clients whether working or enjoying domesticity was also benefitted from her application of chiropractic techniques, optimizing musculoskeletal wellbeing.
Abstract:
This study investigated the effect of weight bearing on joint and limb segment angles in forelimbs and hind limbs of the ridden elephant. Five sound working safari elephants completed walking trials on firm level ground under eight different rider conditions; no rider, one, two and three riders, all with and without saddle. Self-adhesive retro-reflective markers were attached at ten anatomical locations; five on the left forelimb and five on the left hind limb. Digital video cameras recorded and data was analyzed using 2D motion-analysis software. Forelimb and hind limb segment angles (relative to vertical) and joint angles (degrees) were measured at mid stance (MS); range of motion (ROM, degrees) from touchdown to toe off was calculated for each valid (n=970) stride. Data was analyzed by ANOVA and T-Test statistics. The presence of riders significantly influenced (P<0.01) a greater number of limb segments and joint angles in MS than the presence of a saddle. Riders or saddle had no significant effect (P>0.05) on MS angles of manus, carpus, pes or femorotibialis/femoropatellaris joint. At MS, humerus angle of retraction increased with rider number; tarsus joint angle reduced as rider number increased with no saddle with saddle, there was no significant effect. Carpus joint ROM reductions were significant (p<0.001) for 3 riders with and without a saddle. The presence of riders influences limb kinematics in elephants indicating compensation for weight bearing and the potential for musculoskeletal compromise. The use of a saddle may modify the potential for musculoskeletal compromise.
Malgorzata Jennes
Ghent University
Belgium
Title: Lymphocyte subsets activation by Toxoplasma gondii antigens
Time : 16:40-17:00
Biography:
Malgorzata Jennes has obtained Master’s degree in Veterinary Science from the Faculty of the Veterinary Medicine at Ghent University, Belgium. Currently she is pursuing Doctoral studies in the Laboratory of Immunology at the same faculty, where she is also working as a Teaching Assistant.
Abstract:
Toxoplasma gondii is an intracellular parasite, causing a severe disease in immunocompromised humans and having an economic impact in domestic animals. One of the transmission routes to humans is the consumption of raw or undercooked meat from livestock, such as pork. Therefore, an experimental infection study was performed to identify the immunological parameters in pigs, induced by the innate or adapted immune system of the host and to associate the immune response with the presence of the parasite in the tissues. In the present study two groups of seronegative 5-week-old pigs were inoculated at d0 with 6000 tissue cysts of T. gondii strain IPB-LR or IPB-Gangji. The PBMC’s were collected two-weekly and cultured for 72 hours upon in vitro stimulation with heterologous antigens from RH-strain, fractionated by continuous-elution electrophoresis into separate pools. The cells were triple-stained for the flow cytometry and based on the expression of the membrane markers, divided into T-lymphocyte subsets (CD3+CD4+CD8α-, CD3+CD4+CD8αdim and CD3+CD4-CD8αbright), followed by the intracellular IFN-γ staining. Additionally, the supernatant from the cultured cells was tested in IFN-γ ELISA. Four or six months post infection the animals were euthanized and the parasite was detected in tissues by bio-assay and qPCR. In our study we detected a strong increase in the cytokine production, induced by the isolated fractions of the native antigens and confirmed by flow cytometry and cytokine ELISA. Interestingly, this increase was time and strain dependent, being related with the amount of the parasitic DNA detected in the tissue samples.
Rebecca Stroud
McTimoney College of Chiropractic
UK
Title: A preliminary study to investigate the prevalence and progression of pelvic axial rotations among neonate foals
Time : 17:00-17:20
Biography:
Rebecca Stroud has completed her MSc (Animal Manipulation) from the McTimoney College of Chiropratic in 2015. She currently works in private practice as a McTimoney Animal Manipulator and Massage Therapist in Herefordshire, UK. In addition to obtaining her BSc in Equine Science, she has spent over 13 years working in the equine industry. Holding a variety of roles in different equestrian disciplines including work with Grand Prix dressage horses and International Eventers has allowed her to gain valuable experience and knowledge. She is also a regular contributor to the national magazine Horse on the subjects of training, health and welfare.
Abstract:
This study aims to identify the presence or absence of pelvic axial asymmetry in the neonate foal and its progression during the first nine weeks of life using quantitative data. Measurement of the tuber coxae heights in relation to level ground were taken of ten healthy subjects at three time periods: 0-1 week; 4-5 weeks and 8-9 weeks of age. A novel method of measurement was used in the form of two laser measures applied simultaneously. Between the first and second data collection foals received their first turnout. A questionnaire provided qualitative data in order to analyze potentially influential variables. Symmetry indices (SI) of datasets were calculated and analyzed by statistical analysis. There was a significant presence of axial rotation of the pelvis within 0-1 week of age (mean SI=0.337±0.25; p=0.002). These asymmetries did not change significantly between week 0-1 and week 8-9. There was no significant difference of asymmetry between week 0-1 and week 4-5 suggesting turnout did not have an effect on the prevalence of pelvic misalignments in foals. Foals of mares that gave birth standing up displayed significantly greater asymmetry of the pelvis during week 0-1 when compared to foals of mares that gave birth in a recumbent position (mean SI±SD: Recumbent n=8; 0.2497±0.659; standing n=2; 0.686±0.4798; p=0.04). This study shows evidence of significant pelvic axial asymmetry from birth to 8-9 weeks of age in foals.
Bahar Onaran
Ankara University
Turkey
Title: Prevalence of Listeria monocytogenes in cattle and sheep carcasses and presence of L monocytogenes lytic bacteriophages in slaughterhouse wastewater
Time : 17:20-17:40
Biography:
Bahar Onaran is currently a Research Assistant in Ankara University Faculty of Veterinary Medicine, Department of Food Hygiene and Technology. She is interested especially in food microbiology.
Abstract:
Listeria monoctogenes (LM) is a zoonotic food-borne bacterium that leads to a variety of serious infections in humans and those suffering with listeriosis occur in approximately 30% mortality. Epidemiologic studies have revealed that a significant proportion of cases of listeriosis caused by contaminated foods. The use of specific virulent bacteriophages for LM in order to reduce LM load in foods of animal origin before, during and after slaughter processes emerges as an important method. It is reported that the usage of specific virulent bacteriophages to LM as a biocontrol and decontamination agent of LM in foods, do not cause any side effects in humans. This study was aimed to find out the prevalence of LM in cattle and sheep carcasses in slaughter level and isolate virulent LM phages from slaughterhouse wastewater in Ankara and Kirikkale, Turkey. For these purposes, 50 cattle and 80 sheep carcass sponge swap samples were collected from three cattle-sheep slaughterhouses between July 2015 and February 2016 to isolate LM. Besides, 40 slaughterhouses wastewater were taken to detect LM lytic bacteriophages. Two out of the cattle and three of the sheep carcasses were contaminated with LM. Additionally, a total of 6 LM 1/2a and/or 4b lytic phages from 3 out of 40 wastewater samples were isolated. The results showed that 4.0% of cattle and 3.8% of sheep carcasses are contaminated with LM. Therefore, after characterization of listeriophage isolates which have lytic effect on 1/2a and/or 4b serotypes will use for the biocontrol of LM in different food models.
Umesh Kumar Shandilya
National Bureau of Animal Genetic Resources
India
Title: Matrix-based three-dimensional culture of buffalo mammary epithelial cells showed higher induction of genes related to milk protein and fatty acid metabolism
Time : 17:40-18:00
Biography:
Umesh Kumar Shandilya has completed his MSc in Biochemistry from Maharshi Dayanand University and PhD from National Dairy Research Institute, India. He has been awarded with Gold Medal for MSc program and INSPIRE Fellowship for PhD program. He is presently working as DST-SERB Young Scientist at National Bureau of Animal Genetic Resources, Haryana. India. He has published 27 papers in reputed journals.
Abstract:
Demanding transcriptomic studies in livestock animal species could be replaced by good in vitro models mimicking the function of mammary gland. Mammary epithelial cells (MEC) are the functional unit of the mammary gland. Extracellular matrix is known to be a key factor providing normal homeostasis in three-dimensional (3D) environment as important signals are lost when cells are cultured in two-dimensional (2D) environment. The aims of this study were to establish buffalo mammary epithelial cells (BMECs) in 3D culture using extracellular matrix and to determine whether such a 3D culture model has different expression pattern than 2D counterpart. The purified MEC generated after several passages were used to establish 3D culture using Geltrex matrix. The expression of milk casein genes viz., alpha S1-casein (CSN1S1), alpha S2-casein (CSN1S2), beta-casein (CSN2), kappa-casein (CSN3); and fatty acid metabolism genes viz., butyrophilin (BTN1A1), glycerol-3-phosphate acyltransferase (GPAM), fatty acid-binding protein 3 (FABP3), and stearoyl-CoA desaturase (SCD) was assessed in 3D culture in comparison to traditional monolayer culture using qRT-PCR. Notable morphological differences were observed for BMECs grown in 3D culture in comparison to 2D culture. Morphologically, epithelial structures grown in Geltrex matrix (3D) environment showed enhanced functional differentiation in comparison to 2D culture. In 3D culture, lumen and dome-like structures were formed by day 5, whereas polarized acinus-like structure were formed within 15 days of culturing. The expression data showed higher mRNA induction of milk casein and fatty acid metabolism genes in 10-day-old 3D BMECs culture in comparison to 2D monolayer culture. The result suggests that 3D organization of epithelial cells has favorable affection induction of milk and fatty acid metabolism-related genes. Therefore, matrix-based 3D culture of MEC that recapitulate the structural and functional context of normal tissues could provide a better in vitro model to understand the mammary gland functioning of buffaloes.