Sulaiman Depamede
University of Mataram
Indonesia
Title: Pancreatic adenocarcinoma up-regulated factor (PAUF) in the saliva of Bali cattle (Bos javanicus): A biomarker candidate for infections
Biography
Biography: Sulaiman Depamede
Abstract
The aim of this study was to explore the potential of a peptide, pancreatic adenocarcinoma up-regulated factor (PAUF) expressed in the saliva of Bali cattle (Bos javanicus), which was first reported by Depamede (2013), as a biomarker for infections. For this purpose, a polyclonal antibody against PAUF peptide was produced using a synthetic PAUF peptide. This was synthesized from the PAUF amino acid sequence as the results of MALDI-TOF/TOF.MS and bioinformatics analysis of the Bali cattle saliva. Antibodies obtained were used to analyze for the presence of PAUF in Bali cattle (Bos javanicus) saliva as well as in the saliva of the New Zealand dairy cows (Bos taurus). The results showed that PAUF is present at greater abundance in Bali cattle saliva compared to that of Bos taurus. Interestingly, PAUF was found in the milk of infected New Zealand cows (Bos taurus), suggesting that it could also be a marker for mastitis. These results provide a novel potential breed and disease biomarker that could help in better management of Indonesian cattle. These findings still need to be further investigated for their utility as biomarkers as well as the role they play in physiology of the immune response in Bali and New Zealand cattle.