De Bastiani Grasiela
Universidade Federal de Santa Maria
Brazil
Title: Histochemistry of equine damaged tendons and ligaments
Biography
Biography: De Bastiani Grasiela
Abstract
The repair process of tendons and ligaments includes different phases such as inflammation, neovascularization, proliferation of granulation tissue and fibrosis tissue. Collagen type and tissue characteristics of tendon and ligament repair are described. Samples of equine tendons and ligaments specimens of the metacarpophalangeal joint region were evaluated by ultrasonography and macroscopicaly and prepared for routine histopathology. The Masson’s trichromic and pricosirius staining techniques were also performed. Ligaments and tendons pathologic findings included fibroplasia, collagenolysis, cartilaginous metaplasia and lymphohistioplasmacytic inflammation. Tendons and ligaments scars were composed predominantly of type III collagen but there also was some type I collagen. Fiber alignment of tendons and ligaments in the reorganization tissue was not perfect and the appearance was characterized by a lack of the fiber crimp and parallelism. The microscopic and histochemistry staining techniques allowed the characterization of fiber alignment, collagen type, inflammatory cell infiltration and neovascularization that happens during the repair process of tendons and ligaments. These findings at different moments of the repair process were associated with ultrasonographic and anatomopathological findings.