P Sankar
Tamil Nadu Veterinary and Animal Sciences University
India
Title: Effects of novel water soluble nanocurcumin on arsenic-induced genotoxicity in rats
Biography
Biography: P Sankar
Abstract
Curcumin loaded PLGA nanoparticles (CUR-NP) were synthesized to explore whether nanoformulation of curcumin can cause better protective effect than free curcumin against arsenic-induced genotoxicity in rat model. The CUR-NP prepared by emulsion technique was spherical in shape with particle size of 130.8 nm and 86.5% entrapment efficiency. The CUR-NP showed biphasic release pattern and it was dissolved in water easily. Rats were randomly divided into five groups of six each. Group I was kept as the control. In Group II, rats were exposed to sodium arsenite (25 ppm) daily through drinking water for 42 days. Groups III, IV and V were treated with arsenic as in Group II, however, they were administered empty nanoparticle, curcumin (100 mg/kg bw) and CUR-NP (100 mg/kg bw), respectively, by oral gavage during the last 14 days of arsenic exposure. At term, bone marrow cells were collected. The result indicated that arsenic significantly increased the formation of chromosomal aberrations, micronuclei and DNA damage in rat bone marrow cells. Free curcumin and CUR-NP treatment significantly attenuated these arsenic-mediated genotoxicity. However, the magnitude of the effects indicates that CUR-NP has better protective effect than free curcumin at the equivalent dose level for preventing arsenic-induced toxicity.
