3^rd International Veterinary Congress August 18-20, 2016 London, UK

Vlad Vuta is currently a PhD student at the University of Agronomic Study and Veterinary Medicine, Faculty of Veterinary Medicine, Bucharest. He is working at the Institute for Diagnosis and Animal Health Bucharest, Virology Department and he is also a Coordinator of National Reference Laboratory for Rabies. He has more than 30 papers and communications in reputed journals and international congresses.

Rabies is a fatal zoonotic viral disease causing more than 70,000 human deaths each year and is produced by a Lyssavirus. In Romania foxes are the main wildlife reservoir. Oral rabies vaccination (ORV) of this specie is the most effective method to control and eradicate rabies. Supported by co-financing program between Romania and European Union, successive ORV campaigns were conducted. From 2015 to 2017 a multiannual program of ORV is performing. The vaccination area of this study involved of the entire Romanian territory (237.500 km2). The vaccination of foxes is carried out by air distribution of baits from 8 aircraft (number of 5325200 baits with an approx. 25 baits/km2), with a distance between flight lines of 500 meters and 150 meters altitude by avoiding the territories of localities, water surfaces, highways, etc. Estimated surface suitable for aerial vaccination is approximated at 213.375 square kilometers. Around localities and areas difficult to reach by plane it is done at manual distribution (number of 75400 of baits, approximately 25 baits per km2). The data are recorded on Geographical Identification System (GIS) using Geographical Positioning System (GPS). At a 45 days following vaccination campaign, there shall be performed the hunting of foxes in order to assess the efficiency of vaccination; for this purpose, there shall be shot 4 foxes/year/100 km2. Samples of tooth and surrounding alveolar bone are tested by specific fluorescence to detect tetracycline deposits. Immune response is assessed using the indirect enzyme-linked immunosorbent assay (ELISA) method. All positive samples to rabies antigen by FAT technique (Fluorescent Antibody Test) are tested in order to discriminate between wild and vaccinated strains using molecular biology techniques.

Ruta Budreckiene has completed her Bachelor of Chemical Engineering Sciences in 1996, Master’s degree in Polymer Chemistry Sciences in 1998 and Doctor of Physical Sciences (Chemistry) in 2005 from Kaunas University of Technology. She is an Expert of European Food Safety Authority in Lithuanian Republic in field of Panel on Additives and Products or Substances used in Animal Feed and Member of Editorial Board of “Journal of Elementology”. She is currently an Associate Professor at the Department of Biochemistry, Faculty of Medicine at Lithuanian University of Health Sciences.

The majority of cosmetics for dogs are shampoos. These are used not only to wash the coat and improve its structural properties but also as adjuvant treatment in various skin diseases. The dogs’ owners in Lithuania are beginning carefully to take care of their pet well-being, skin health and appearance of the coat. There are many cosmetic devices for this reason and the market is expanding by the addition of new products. So, the purpose of this work was to evaluate shampoos effects to dogs’ hair for daily coat care. There were three dogs in the research work involved and three shampoos for daily coat care for research work selected randomly: X1, X2 and X3. Some hair of each dogs were used for analysis by Scanning Electron Microscope FEI Quanta 200 FEG. There were taken photos of hair cross section. Other hairs were used to evaluate hair ratio of convolution. After the research, there were established that which exploratory shampoo is the most economical (X3) and which is the most suitable for dogs’ skin pH (X3). Also, the influence of shampoos’ for hair surface, cross section diameter and ratio of convolution was observed. In conclusion, the maximum visible changes were observed in photos after bathing with X3 shampoo, which was evaluated like the best by the dogs’ owners.