University of Tehran, Iran
Title: Study effects of Ivermectin and interaction with supplements vitamin A and vitamin C on reproduction system in male wistar rat
Biography: Alireza Bashiri
Objective: Ivermectin is an antiparasitic drug with a widespread activity on internal and external parasites. The purpose of this study was to study effects of Ivermectin on sperm quality and quantity on adult male wistar rats.
Material and Method: For an invivo examination, a total of 25 male wistar rats of 6-8 weeks old and 150-200g, were equally divided into five experimental groups; control group; oral solution of Ivermectin; mixture of Ivermectin and vitamin A; mixture of Ivermectin and vitamin C; mixture of Ivermectin, vitamin A and vitamin C; once a week for three weeks. At the end of the study, the rats were sacrificed and sperm analysis were done. For investigation of motility percentage, first percent of motile sperms were observed in several microscope fields. The average of them as the percent of motility was registered. Sperm viability was done via microscopic observation and eosin stain. Then ratio of alive sperms to whole sperms were studied. Sperm count was done through neobar lam. The speed of sperms was determined via reverse microscope and homocytometer lam.
Results: Agreeing with results, in group received Ivermectin the level of motility in comparison with control group decreased. In addition, in groups treated with vitamin A or vitamin C and also combination of both vitamins A and C, the level of motility increased. This increase in group received both vitamins A and C comparing with group treated with Ivermectin only, was significant. Findings of this study have shown that in groups treated with free Ivermectin the level of viability decreased. According to the results, in groups treated with vitamin A or vitamin C and group that treated with both vitamins A and C the level of viability comparing with group received Ivermectin only increased. This increase in group received both vitamin A and vitamin C compared with group treated with free Ivermectin was significant. As results shown in group received both vitamin A and vitamin C in comparison with group treated with free Ivermectin, the level of sperm count increased significantly. In the study of sperm count, group treated with Ivermectin has shown reduce in this factor compared with control group.
Conclusion: It can be concluded that the use of free Ivermectin has side effects on fertility rate in male rats, while consumption of vitamin A and vitamin C act as two reproduction system’s amplifier.