Gulay Ciftci
Ondokuz Mayis University, Turkey
Title: The investigation of the effects of cholesterol and Lactobacillus acidophilus on some biochemical parameters in rats
Biography
Biography: Gulay Ciftci
Abstract
Statement of the Problem: The investigation of the effects of the administration of Lactobacillus acidophilus to rats fed with 2% cholesterol on the changes in total protein (TP), albumin (Alb), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), oxidized low-density lipoprotein (Ox-LDL) and high-density lipoprotein (HDL) was aimed.
Methodology & Theoretical Orientation: The animal material comprised of 30 adult-male-Sprague-Dawley-rats. Rats were divided into three groups. Control group (C) was fed with standard rat food for 8 weeks. Hypercholesterolemic group (HC) was fed with a ration comprising of the food with 2% cholesterol for 8 weeks. Hypercholesterolemic and probiotic-administrated group (HCL) was fed with a ration comprising of food with 2% cholesterol for 8 weeks and fed orally with 2x108 cfu/ml/day L.acidophilus probiotic for the last 4 weeks of the trial. At the end of 8 weeks, the serum levels of TP, Alb, TC, TG, LDL and HDL were detected by auto-analyzer. The level of Ox-LDL was measured by ELISA in brain supernatants.
Findings: The slightly increase of TP level (p>0.05) in HC group was determined compared to control group. In HCL group, this level slightly decreased (p>0.05). The changes in albumin level among the groups were not significant (p>0.05). It was detected that TC level in HC group increased (p<0.05); but in HCL group, it decreased and approached to the level of control group (p<0.05). The TG level in HC group increased compared to control group (p<0.05); but in HCL group, it decreased compared to HC group (p<0.05). It was determined that the level of HDL in HC group decreased compared to control group (p<0.05), but increased in HCL group (p>0.05). The changes in Ox-LDL level in HC group was determined as increased compared to control group (p<0.05); but in HCL group, it decreased compared to HC group (p<0.05).
Conclusion & Significance: It was thought that the administration of Lactobacillus acidophilus as a probiotic might be useful in reducing the cholesterol level.