Tofazzal Md. Rakib
Chittagong Veterinary and Animal Sciences University, Bangladesh
Title: revalence of methicillin/oxacillin resistant staphylococcus aureus (MRSA) in stray and pet dogs of Chittagong metropolitan area, Bangladesh
Biography
Biography: Tofazzal Md. Rakib
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) is a multidrug resistant organism that threatens the effectiveness of antibiotics worldwide. The study was carried out in stray (n=108) and pet (n=50) dogs in Chittagong Metropolitan area, Bangladesh to determine the proportionate prevalence of MRSA strains by molecular techniques and their in-vitro antibiotic susceptibility patterns. Oral swabs (N=158) were collected using sterile cotton swabs and were isolated according to cultural properties and molecular amplification of nuc gene. The isolates were subjected to antimicrobial sensitivity testing by Kirby-Bauer method, and resistant mecA gene of MRSA was detected by polymerase chain reaction (PCR). 37 (34.91%) stray and 17 (34%) pet dogs were found positive for S. aureus, of which 18 (16.98%) stray and 7 (14%) pet dogs were found positive for nuc gene of S. aureus. Highest prevalence of MRSA was found in Bayezid (57.89%) and Kotwali (100%), whereas lowest in Pahartoli (22.22%) and Chandgaon (0%) for stray and pet dogs, respectively. A high percentage of MRSA isolates were resistant to antibiotics, including oxytetracycline (83.33%), azithromycin (77.78%), oxacillin (75.93%), sulfamethoxazole + trimethoprim (74.07%), methicillin (74.07%), ciprofloxacin (74.07%), gentamicin (74.07%), amoxicillin (46.3%) and ceftriaxone (42.59%). So, it can be concluded that MRSA in dogs are prevalent in Chittagong, Bangladesh.