Harikrishna Pillai
Indian Veterinary Research Institute, India
Title: Regucalcin in buffalo fetal fibroblasts and wharton’s jelly stem cells
Biography
Biography: Harikrishna Pillai
Abstract
Statement of the problem: Regucalcin/Senescence Marker Protein-30 (RGN/SMP-30), a multifunctional calcium binding protein, has many pivotal roles in calcium homeostasis, ascorbic acid biosynthesis and having anti-prolific and anti-apoptotic functions. All these functions are very important in stem cells. However, there are no reports regarding the role of RGN in stem cells. The aim of the present study was to investigate the expression of RGN in buffalo derived Wharton’s jelly (BWJ) and fetal fibroblast (BFF).
Methodology: Gravid buffalo uteri of (~40- 50 days old) were procured. Mesenchymal stem cells (MSCs) were isolated from BFF and BWJ and cultured in DMEM with 15% FBS. The expression of RGN in BFF and BWJ was elucidated by qPCR, immunostaining and western blot analysis.
Results: The real-time PCR products of BWJ and BFF were confirmed on 2% agarose gel electrophoresis by the presence of 197bp product. An immunoreactive band of 34 kDa specific for RGN was detected in Western blot of BWJ and BFF. Cellular localization in both BWJ and BFF by immunocytochemistry revealed that RGN is located in both cytoplasm and nucleus.
Conclusion & Significance: This study is the first report on the expression of RGN in stem cells of any kind. It confirms the presence of RGN in fetal stem cells in mRNA and protein level. As this work provides initial information regarding the presence of RGN in MSCs, further investigation is needed to find out the role of RGN in important role in the differentiation, proliferation and aging of stem cells.